Investigation of Toxic Effects of Putrescine A. Determination of Ld<Sub>50</sub> Level of Putrescine With Intraperitoneal Applied To Mice | ICRES

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Title

Investigation of Toxic Effects of Putrescine A. Determination of Ld50 Level of Putrescine With Intraperitoneal Applied To Mice

Authors

Dr. Ali Nazmi Can Dogan, Gole State Hospital, Internal Medicine Clinic, Ardahan, Turkey
Assist. Prof. Dr. Dincer Erdag, Kafkas University Vocational School of Health Services, Kars, Turkey

Abstract

In this study, it was aimed to calculate the LD50 level of putrescine administered intraperitoneally to mice. Putrescine is synthesized from the arginine by bacteria in the body and in the intestines after death. It can also be obtained synthetically. In this study, 20 ± 2 g male rats belonging to the Mus musculus albino breed were used. Mice were divided into 7 groups, each consisting of 10 groups. During the experiment, ad libitum was fed with standard diet and tap water. Solutions of putrescine were prepared with 500, 600, 700, 800, 900, 1000 and 1100 mg/5 mL commercial saline respectively. Each group was ip administered from the prepared solution at 1500, 1800, 2100, 2400, 2700, 3000 and 3300 mg/kg dose (0.3 mL volume) respectively. Animals were monitored twice a day and those who died within 14 days were noted. The LD50 value of putrescine was calculated using the Behrens-Kerber method. As a result of the study, the LD50 dose of putrescine in mice was determined as 2175 mg/kg in intraperitoneal administration. It is suggested that these findings should be taken into consideration in their studies with putrescine.

 Keywords

polyamine, putrescine, mouse, ld50  

Citation

Dogan, A.N.C. & Erdag, D. (2021). Investigation of Toxic Effects of Putrescine A. Determination of Ld50 Level of Putrescine With Intraperitoneal Applied To Mice. In M. Shelley, I. Chiang, & O. T. Ozturk (Eds.), Proceedings of ICRES 2021--International Conference on Research in Education and Science (pp. 129-136). Monument, CO, USA: ISTES Organization. Retrieved 26 October 2021 from www.2021.icres.net/proceedings/134/.

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